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| |  | Cat. No. AP1046 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids at the C-terminus of human CtIP | | Form: Liquid | | Formulation: In Tris citrate/phosphate buffer, pH 7-8. | | Preservative: ≤0.1% sodium azide | | Positive Control: MCF7 cells | | Comments: Recognizes the ~125 kDa CtIP protein in MCF7 cells. | | Ref.: Chen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535. Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478. Germani, A., et al. 2003. Oncogene 22, 8845. Li, S., et al. 2000. Nature 406, 210. Wong, A.K., et al. 1998. Oncogene 17, 2279. |
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Lane 1: Detection of CtIP by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF7 cells. Primary antibody: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (1 µg/ml). Detection: chemiluminescence. Lane 2/3: Detection of CtIP by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from MCF7 cells. Antibodies for IP: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (3 µg/mg protein) (lane 2) and Rabbit IgG (3 µg/mg protein) (lane 3). Immunoblotting conditions: same as above.
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