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| | Anti-Hsp90a Mouse mAb (EMD-17D7) |
|  | Cat. No. CA1023 |
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Anti-Heat Shock Protein 90a | Host: Mouse | | Isotype: IgG1 | | Immunogen: Hsp90a, His•Tag®, Human, Recombinant (Cat. No. 385901) | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤0.1% sodium azide | | Positive Control: H460 and HeLa cells | | Comments: Recognizes the ~90 kDa Hsp90a protein in HeLa cells. Does not recognize Hsp90b. | | Ref.: Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297. Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179. Nemoto, T., et al. 1998. Biochem. J. 330, 989. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| EMD-17D7 | human | ELISA, IB, IP
See Key |
Detection of human Hsp90a by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 mg) from H-460 cells. Antibody for IP: Anti-Hsp90a Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 mg/700 mg protein). Conditions for immunoblotting: Sample: 10 ml of 50 ml Hsp90a IP (above). Primary antibody: Anti-Hsp90a Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
Detection of human Hsp90a by immunoblotting. Samples: Whole cell lysate (50 mg) from HeLa cells (lane A), Hsp90b, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90a, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90a Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 mg/ml). Detection: chemiluminescence.
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