Detection of human Hsp27 by immunocytochemistry. Sample: HeLa cells fixed with 100% methanol. Primary antibody: Anti-Hsp27 Mouse mAb (EMD-35) (Cat. No. CA1025) (3 mg/ml). Detection: fluorescence with DAPI counterstain.
Detection of human Hsp27 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (700 µg) from HeLa cells. Antibody for IP: Anti-Hsp27 Mouse mAb (EMD-35) (Cat. No. CA1025) (5 µg/700 µg protein). Conditions for immunoblotting: Sample: 10 ml of 50 ml Hsp27 IP (above). Primary antibody: Anti-Hsp27 Rabbit pAb (Cat. No. CA1025) (1:5000). Detection: chemiluminescence.
Detection of human Hsp27 by immunoblotting. Samples: Hsp27, His•Tag®, Human, Recombinant (Cat. No. 385909) (100 ng, lane A) and whole cell lysate (50 mg) from HeLa cells (lane B). Primary antibody: Anti-Hsp27 Mouse mAb (EMD-35) (Cat. No. CA1025) (1 mg/ml). Detection: chemiluminescence.
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