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| | Anti-N-Cadherin Mouse mAb (13A9) |
|  | Cat. No. CA1029 |
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| Host: Mouse | | Isotype: IgG | | Immunogen: a recombinant fusion protein consisting of the cytoplasmic domain of human N-cadherin, expressed in E. coli | | Form: Liquid | | Formulation: Undiluted ascites. | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa cells | | Comments: Recognizes the ~140 kDa N-cadherin protein in HeLa cells. | | Ref.: Christofori, G. 2006. Nature 44, 444. Cvallaro, U., et al. 2006. Exp. Cell Res. 312, 659. Cowin, P., et al. 2005. Curr. Op. Cell Biol. 17, 499. |
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Lane 1: Detection of human N-cadherin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-N-Cadherin Mouse mAb (13A9) (Cat. No. CA1029) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human N-cadherin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti N-Cadherin Mouse mAb (13A9) (Cat No. CA1029) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1.
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