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| | PhosphoDetect™ Anti-Caveolin-2 (pSer23) Rabbit pAb |
|  | Cat. No. CB1005 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide (SYSHHpSGLEYADPE) corresponding to amino acids 18-31 of human caveolin-2 | | Form: Liquid | | Formulation: In PBS, 50% glycerol, 1 mg/ml BSA. | | Preservative: ≤0.1% sodium azide, 0.01% thimerosal | | Positive Control: LNCaP cells expressing caveolin-2 | | Comments: Recognizes the ~20 kDa caveolin-2 protein phosphorylated on Ser23 in LNCaP cells expressing caveolin-2. | | Ref.: Sowa, G., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 6511. Mora, R., et al. 1999. J. Biol. Chem. 274, 25708. Scherer, P.E., et al. 1996. Proc. Natl. Acad. Sci USA 93, 131. |
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* Additional Product Information
Detection of human caveolin-2 by immunoblotting. Sample: Whole cell lysate (30 µg total protein) from LNCaP cell lysates expressing caveolin-2 incubated without (-, lane 1) or with (+, lane 2) calf alkaline phosphatase (CIP). Primary antibody: Anti-Caveolin-2 (18-31) Rabbit pAb (Cat. No. CB1004) (top panel), PhosphoDetect™ Anti-Caveolin-2 (pSer23) Rabbit pAb (Cat. No. CB1005) (middle panel), and PhosphoDetect™ Anti-Caveolin-2 (pSer36) Rabbit pAb (Cat. No. CB1006) (bottom panel). Image kindly provided by Dr. William Sessa of Yale University.
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