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Kit Resource
  InnoZyme™ Cathepsin B Activity Assay Kit, Fluorogenic
Cat. No. CBA001  
All Categories » Calbiochem » Kits and Tools » Protease Assays » Cathepsin Assays

Format: 96-well plate
Form: 96 Tests
Detection Method: Fluorometric
Sensitivity: 0.63 ng/ml
Assay Range: 0.09 - 100 ng/ml
Assay Time: 30 min
Sample Type: Cells and tissues
Positive Control: Cathepsin B
Kit Contents: Cathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol.
Comments: A sensitive fluorogenic assay for the measurement of cathepsin B activity (Excitation max: ~360-380 nm; Emission max: ~430-460 nm). Cathepsin B is a cysteine proteinase which has been associated with increased invasivessness and development of the malignant cell phenotype. Cathepsin B has also been implicated in inflammatory airway disease.
Ref.: Bervar, A., et al. 2003. Biol. Chem. 384, 447. Grigolo, B. et al. 2003. Biomaterials 24, 1751. Scolaris, A., et al. 2002. Biol. Chem. 383, 1297. Murata, M., et al. 1991. FEBS Lett. 280, 307. Berquin, I.M. and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281. Burnett, D., 1995. Arch. Biochem. Biophys. 317, 305. Reddy, V.Y., et al. 1995. Proc. Natl. Acad. Sci. 92, 3849. Buttle, D.J., 1994. In: Immunopharmacology of Joints and Connective Tissue (Dingle, J.T. and Davies, M.E., eds) London: Academic Press. 225. Barrett, A.J. and Kirschke, H., 1981. Methods Enzymol. 80, 535.

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Standard Curve. Calibration curve generated by various dilutions of the free AMC standard included in the kit (above).

Related Literature:

Antibodies for Cancer Research Product Guide

Material Safety Data Sheets:
CBA001: InnoZyme™ Cathepsin B Activity Assay Kit, Fluorogenic - English
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