| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Fluorometric | | Sensitivity: 0.63 ng/ml | | Assay Range: 0.09 - 100 ng/ml | | Assay Time: 30 min | | Sample Type: Cells and tissues | | Positive Control: Cathepsin B | | Kit Contents: Cathepsin B Enzyme, Calibration Standard, Cathepsin B Substrate, Assay Buffer, Cathepsin B Inhibitor, Reduction Reagent, Cell Lysis Buffer, Microtiter Plate, Plate Sealer, and a user protocol. | | Comments: A sensitive fluorogenic assay for the measurement of cathepsin B activity (Excitation max: ~360-380 nm; Emission max: ~430-460 nm). Cathepsin B is a cysteine proteinase which has been associated with increased invasivessness and development of the malignant cell phenotype. Cathepsin B has also been implicated in inflammatory airway disease. | | Ref.: Bervar, A., et al. 2003. Biol. Chem. 384, 447. Grigolo, B. et al. 2003. Biomaterials 24, 1751. Scolaris, A., et al. 2002. Biol. Chem. 383, 1297. Murata, M., et al. 1991. FEBS Lett. 280, 307. Berquin, I.M. and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281. Burnett, D., 1995. Arch. Biochem. Biophys. 317, 305. Reddy, V.Y., et al. 1995. Proc. Natl. Acad. Sci. 92, 3849. Buttle, D.J., 1994. In: Immunopharmacology of Joints and Connective Tissue (Dingle, J.T. and Davies, M.E., eds) London: Academic Press. 225. Barrett, A.J. and Kirschke, H., 1981. Methods Enzymol. 80, 535. | |