Protein Kinase B, Phospho-Specific (Thr308) ELISA Kit | Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: ≤0.8 units/ml | | Assay Range: 1.6-100 units/ml | | Assay Time: 4 h | | Sample Type: Cell lysates | | Positive Control: Akt (pThr308) | | Kit Contents: Coated 96-Well Plate, Akt Phospho-Thr308 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | | Comments: Detects Akt when phosphorylated on Thr308. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat. | | Ref.: Kops, G.J., et al. 2002. Mol. Cell Biol. 22, 2025. Stiles, B., et al. 2002. Mol. Cell Biol. 22, 3842. Sun, M., et al. 2001. Am. J. Pathol. 159, 431. Fulton, D., et al. 1999. Nature 399, 597. Datta, S.R., et al. 1997. Cell 91, 231. Alessi, D.R., et al. 1996. EMBO J. 15, 6541. Bellacosa, A., et al. 1995. Int. J. Cancer 64, 280. Burgering, B.M. and Coffer, P.J. 1995. Nature 376, 599. Franke, T.F., et al. 1995. Cell 81, 727. Bellacosa, A., et al. 1993. Oncogene 8, 745. Coffer, P.J. and Woodgett, J.R. 1991. Eur. J. Biochem. 201, 475. Jones, P.F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4171. Staal, S.P. 1987. Proc. Natl. Acad. Sci. USA 84, 5034. |
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