Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Species Reactivity: human, monkey, mouse

Sensitivity: ≤0.8 U/ml

Assay Range: 1.6-100 U/ml

Assay Time: 4 h

Sample Type: Cell lysates

Kit Contents: Coated 96-Well Plate, ERK1/2 Phospho-Thr185/Tyr187 Standard, Diluents, Detector antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.

Comments: Detects the dually phosphorylated form of p38 MAP kinase at Thr180 and Tyr182.This activation occurs in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, LPS, and UV light. Although this kit was designed for human samples, it cross-reacts with mouse and monkey.

Ref.: Ge, B., et al. 2002. Science 295, 1291. Browning, D. D., et al. 2000. J. Biol. Chem. 275, 2811. Ono, K., and Han, J. 2000. Cell Signal. 12, 1. Hale, K. K., et al. 1999. J. Immunol. 162, 4246. Wang, X.S., et al. 1997. J. Biol. Chem. 272, 23668. Han, J., et al. 1996. J. Biol. Chem. 271, 2886. Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531. Han, J., et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Ershler, M., et al. 1993. Gene 124, 305.

R: 21/22-36/38; S: 36/37-45