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 | 2 to 8°C |  | Blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | InnoCyte™ Cell Migration Assay, 96-well |
|  | Cat. No. CBA010 |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Fluorometric | | Assay Time: 3-5 h | | Sample Type: Adherent cells | | Kit Contents: Sterile 96-Well Migration Chamber, Culture Tray, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol. | | Comments: A convenient assay for the chemotaxis cell migration. Cells migrate through a 8 mm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye (Excitation max.: ~485 nm; Emission max.: ~520 nm). | | Ref.: Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459. Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677. Smilenov, L. B., et al. 1999. Science 286, 1172. Burridge, K., et al. 1997. Trends Cell Biol. 7, 342. Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719. Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359. |
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Assay Characteristics and Examples. ~50,000 human umbilical venous endothelial cells (HUVECs) were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without chemotactic components in the lower chamber for 4 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol. Latrunculin A, a powerful inhibitor of actin polymerization, was added to an aliquot of cell suspension immediately prior to loading the cells in the wells.
Assay Characteristics and Examples. ~60,000 HT-1080 cells were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without serum for 3 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol.
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