| Format: 96-well plate | | Form: 72 Tests | | Detection Method: Fluorometric | | Assay Time: 2.5 h | | Sample Type: Adherent cultured cells | | Kit Contents: Coated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol. | | Comments: A convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent dye. | | Ref.: Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551. Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA96, 2588. Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153. Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476. Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104. Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460. Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York. Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490. | |