Calbiochem: CBA016: InnoZyme™ Human Neutrophil Elastase Immunocapture Activity Assay Kit

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��	InnoZyme™ Human Neutrophil Elastase Immunocapture Activity Assay Kit

Cat. No. CBA016��

All Categories � Calbiochem � Kits and Tools � Protease Assays � Serum Protease Assays

HNE Immunocapture Activity Assay Kit

Format: 96-well plate
Form: 96 Tests
Detection Method: Fluorometric
Assay Range: 0.625-20 ng/ml (4 h) and 62.5-2000 pg/ml (24 h)
Assay Time: 6 h
Sample Type: Cell lysates, body fluids, for screening HNE inhibitors
Positive Control: Human neutrophil elastase
Kit Contents: Anti-HNE-Coated 96-Well Plate, HNE Control, Substrate, AMC Standard, Assay Buffer, Sample Buffer, Plate Sealer, and a user protocol.
Comments: A sensitive and selective assay for the measurement of human neutrophil elastase (HNE), a serine protease with the ability to solubilize fibrous elastin. Anti-HNE is immobilized onto a 96-well plate; activity is measured with a fluorogenic substrate (Ex: 360-380 nm; Em.: 440-460 nm).

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1 kit Y $892.00

AMC Standard. Calibration curve generated with the free AMC standard included in the kit.

HNE Activity. The activity of native HNE, included in the kit, using MeoSuc-Ala-Ala-Pro-Val AMC at pH 7.5 after 4 h incubation at 37�C. Fluorescence reading: excitation 360 nm, emission 440-460 nm.

HNE Activity. The activity of native HNE, included in the kit, using MeoSuc-Ala-Ala-Pro-Val AMC at pH 7.5 after 24 h incubation at 37�C. Fluorescence reading: excitation 360 nm, emission 460 nm.

HNE Activity in Human Plasma. Representation of HNE activity in human plasma assayed with Cat. No. CBA016. Fluorescence reading: excitation 360 nm, emission 460 nm.

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CBA016: InnoZyme™ Human Neutrophil Elastase Immunocapture Activity Assay Kit - English
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