| Format: 24-well plate | | Form: 12 Tests | | Detection Method: Fluorometric | | Assay Time: 3-5 h | | Sample Type: Adherent cells | | Kit Contents: 12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol. | | Comments: A convenient assay for chemotaxis cell migration in 24-well plates. Cells migrate through a 8 mm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye. | | Ref.: Wells, A., et al. 2002. Acta Oncol. 41, 124. Staff, A. C. 2001. Scand. J. Clin. Lab. Invest. 61, 257. Eatock, M.M., et al. 2000. Cancer Treat. Rev. 26, 19. Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459. Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677. Smilenov, L. B., et al. 1999. Science 286, 1172. Burridge, K. et al. 1997. Trends Cell Biol. 7, 342. Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719. Price, J.T., et al. 1997. Crit. Rev. Biochem. Mol. Biol. 32, 175. Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359. | |