Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorometric

Sensitivity: 1.2 ng/ml

Assay Range: 1.56-100 ng/ml

Assay Time: 1.5 - 2 h

Sample Type: Cell lysates, tissues, or purified cathepsin L

Positive Control: Cathepsin L

Kit Contents: Cathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol.

Comments: A highly sensitive and selective fluorogenic assay for the detection of Cathepsin L from human and mouse samples. Cathepsin L is detected with the fluorogenic substrate Z-Phe-Arg-AMC (Exc. max: 360 nm; Emi. max: 460 nm). Interference from Cathepsin B is eliminated by the incorporation of CA 074, a specific cathepsin B inhibitor. This kit is also useful for screening cathepsin L inhibitors.

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