InnoZyme™ MPO Activity Kit | Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Sensitivity: 330 pg/ml | | Assay Range: 5 -100 ng/ml | | Assay Time: 2 h | | Sample Type: Cell lysates, CSF, and serum | | Positive Control: Human myeloperoxidase | | Kit Contents: Anti-MPO Coated 96-Well Plate, MPO Standard, TMB, Hydrogen Peroxide, Assay Buffer, Sample Buffer, Stop Solution, Plate Sealers, and a user protocol. | | Comments: The InnoZyme™ Myeloperoxidase Activity Kit is a specific and sensitive assay designed to measure myeloperoxidase activity and to screen enzyme inhibitors. The assay uses a polyclonal antibody specific for human MPO immobilized on a 96-well plate to specifically capture the enzyme. Activity of captured myeloperoxidase is measured using a detection reagent that includes TMB and hydrogen peroxide. Following color development, the reaction is stopped with sulfuric acid and the absorbance of the oxidized TMB is detected at 450 nm. | | Ref.: Weil, S.C., et al. 1988. Science 240, 790. Winterbourn, C.C., et al. 2000. Curr. Opin. Hematol. 7, 53. Brennan, M.L., et al. 2003. New Engl. J Med. 349, 1595. Zheng, L., et al. 2004. J. Biol. Chem. in press Vita, J.A., et al. 2004. Circulation, 110, 1134. | | Not available for sale in Germany. |
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