Format: 96-well plate

Form: 72 Tests (36 Laminin and 36 BMC)

Detection Method: Fluorometric

Assay Time: 2.5 h

Sample Type: Adherent cultured cells

Kit Contents: Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol.

Comments: The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.

Ref.: Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551. Ekblom, P., et al. 2003. Matrix Biology 22, 35. Li, S., et al. 2002. J. Cell Biol. 157, 1279. Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588. Aumailley, M., et al. 1998. J. Anat. 193, 1. Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104. Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460. Baron von Evercooren, A., et al. 1982. J. Neurosci. Res. 8, 179.