Format: 96-well plate

Form: 72 Tests (36 Gal-1 and 36 Gal-3)

Detection Method: Fluorometric

Assay Time: 2.5 h

Sample Type: Adherent cultured cells

Kit Contents: Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol.

Comments: The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.

Ref.: Takenaka, Y., et al. 2004. Glycoconjugate Journal 19, 543. Horiguchi, N., et al. 2003. J. Biochem. 134, 689. Levy, Y., et al. 2001. J. Biol. Chem. 276, 31285. Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588. Kaltner, H., et al. 1998. Acta Anatomica 161, 162. Perillo, N. L., et al. 1998. J. Mol. Med. 76, 402. Warfield, P. 1998. Biochem. Biophys. Res. Commun 246, 788. Akahani, S., et al. 1997. Cancer Res. 57, 5272. Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460. Barondes, S.H., et al. 1994. J. Biol. Chem. 269, 20907. Ochieng, J., et al. 1994. Biochemistry 33, 14109.