Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Species Reactivity: human, mouse, rat

Sensitivity: ≤1 ng/ml

Assay Range: 1.6 - 50 ng/ml

Assay Time: 4 h

Sample Type: Cell lysates

Kit Contents: Src Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.

Comments: Detects and quantifies the levels of c-Src protein, independent of its phosphorylation state. c-Src is the member of homologous non-receptor tyrosine kinases family with two major phosphorylation sites on Src at Tyr426 and Tyr527. Activation of c-Src is critically involved in carcinoma cell adhesion, migration, metastasis and the enhancement of cell survival. Although this kit is designed for human cell lines, platelets and lymphocytes, it cross-reacts with mouse and rat cells.

Ref.: Laird, A.D., et al. 2003. Mol. Cancer Therap. 2, 461. Mestelin, T. and T. Hunter 2002. Science's STKE www.stke.org/cgi/content/full/OC_sigtrans;2002/115/pe3. Courtneidge, S.A. 2001. Biochem. Soc. Trans. 30, 11. Martin, G.S. 2001. Nat. Rev. Mol. Cell. Biol. 2, 467. Thomas, S.M. and J.S. Brugge 1997Annu. Rev. Cell Dev. Biol. 13, 513.