| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: ≤ 1 Unit/ml | | Assay Range: 1.6-100 Units/ml | | Assay Time: 4 h | | Sample Type: Cells | | Kit Contents: Src (Tyr418) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Detects and quantifies the level of c-Src protein that is phosphorylated at Tyr418. c-Src is the member of homologous non-receptor tyrosine kinases family which is critically involved in carcinoma cell adhesion, migration, metastasis, and the enhancement of cell survival. Although this kit is designed for use with human cell lines, platelets, and lymphocytes, it cross-reacts with mouse and rat cells. | | | | Ref.: Laird, A.D., et al., 2003. Mol. Cancer Therap. 2, 461. Mestelin, T. and T. Hunter, 2002 Science's STKE www.stke.org/cgi/content/full/OC_sigtrans;2002/115/pe3. Courtneidge, S.A., 2001. Biochem. Soc. Trans. 30, 11. Martin, G.S., 2001. Nat. Rev. Mol. Cell. Biol. 2, 467. Thomas, S.M. and J.S. Brugge, 1997. Annu. Rev. Cell Dev. Biol. 13, 513. |
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