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Kinase Kit Resource

Kit Resource

VEGF Signaling Pathway
  p38 MAP Kinase ELISA Kit
Cat. No. CBA029  
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Mitogen Activated Protein Kinase (MAPK)

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, monkey, mouse
Sensitivity: ≤16 pg/ml
Assay Range: 31.2-2000 pg/ml
Assay Time: 4 h
Sample Type: Cells
Kit Contents: Coated 96-Well Plate, p38 MAPK Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.
Comments: Detects and quantifies the levels of p38 MAPK protein independent of its phosphorylation state. p38 MAPK activation occurs in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, LPS, and UV light. Although this kit is designed for human samples, it cross-reacts with mouse and monkey.
Ref.: Ge, B., et al. 2002. Science 295, 1291. Browning, D.D., et al. 2000. J. Biol. Chem. 275, 2811. Ono, K., and Han, J., 2000. Cell Signal. 12, 1. Hale, K.K., et al. 1999. J. Immunol. 162, 4246. Wang, X.S., et al. 1997. J. Biol. Chem. 272, 23668. Han, J., et al. 1996. J. Biol. Chem. 271, 2886. Zervos, A.S., et al. 1995. Proc. Nat'l. Acad. Sci. USA 92, 10531. Han, J. et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Ershler, M., et al. 1993. Gene 124, 305.

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Cat. No.Disclaimers
CBA029
Canadian Export RegulationsDue to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.


Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of p38 MAPK. The data presented in Figure 1 show that the sensitivity of the ELISA is ~10x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-p38 MAPK antibody and, chemiluminescent detection.

Related Literature:

Biologics 32.1

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CBA029: p38 MAP Kinase ELISA Kit - English
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All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Mitogen Activated Protein Kinase (MAPK)