| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, monkey, mouse | | Sensitivity: ≤16 pg/ml | | Assay Range: 31.2-2000 pg/ml | | Assay Time: 4 h | | Sample Type: Cells | | Kit Contents: Coated 96-Well Plate, p38 MAPK Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Detects and quantifies the levels of p38 MAPK protein independent of its phosphorylation state. p38 MAPK activation occurs in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, LPS, and UV light. Although this kit is designed for human samples, it cross-reacts with mouse and monkey. | | Ref.: Ge, B., et al. 2002. Science 295, 1291. Browning, D.D., et al. 2000. J. Biol. Chem. 275, 2811. Ono, K., and Han, J., 2000. Cell Signal. 12, 1. Hale, K.K., et al. 1999. J. Immunol. 162, 4246. Wang, X.S., et al. 1997. J. Biol. Chem. 272, 23668. Han, J., et al. 1996. J. Biol. Chem. 271, 2886. Zervos, A.S., et al. 1995. Proc. Nat'l. Acad. Sci. USA 92, 10531. Han, J. et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Ershler, M., et al. 1993. Gene 124, 305. | |