Return to our Home Page


Tech Resources
User Protocol
MSDS - English
Other Languages
Certificate of Analysis
Store
2 to 8°C2 to 8°C
Ship
Shipped with Blue Ice
<br>
Shipped with blue ice (water ice)!Blue ice
Multiple Toxicity Values, refer to MSDSMultiple Toxicity Values, refer to MSDS
Note: Store and Ship conditions may differ.
See Key

PhosphoDetect™ Phospho-specific Antibodies

Kit Resource

VEGF Signaling Pathway
  PhosphoDetect™ MEK1 (pSer218/222) ELISA Kit
Cat. No. CBA030  
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Mitogen Activated Protein Kinase (MAPK)

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse
Sensitivity: ≤ 0.9 Units/ml
Assay Range: 1.6-100 units/ml
Assay Time: 3 h 20 min
Sample Type: Cells
Kit Contents: MEK1 (Ser218/222) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.
Comments: Detects and quantifies the level of MEK1 protein that is dually phosphorylated at Ser218 and Ser222. MEK1 is a member of the MEK family of dual specificity protein kinases which phosphorylates threonine and tyrosine residues on MAP kinases ERK 1 and 2. Activation of MEK1 is critically involved in a wide range of cellular processes including cell proliferation, differentiation, and apoptosis. This kit is designed for use with human and mouse cells.
Ref.: Colanzi, A., et al. 2003. J. Cell Biol. 161, 27. Slack-Davis, J.K., et al. 2003. Cell Biol. 162, 281. Suthiphongchai, T., et al. 2003. Oncol. Res. 13, 253. Alpin, A.E., et al. 2001. J. Cell Biol. 153, 273. Lee, S.E., et al. 2001. J. Biol. Chem. 276, 49343. Xu, B.E. 2001. J. Biol. Chem. 276, 26509. Kolch, W., et al. 2000. Biochem. J. 351, 289. Bondzi, C., et al. 2000. Oncogene 19, 5030. Whitmarsh, A.J. and R.J. Davis 1999. Science's stke www.stke.org/cgi/content/full/OC_sigtrans; 1999/1/pe1. Widman, C., et al. 1999. Physiol. Rev. 79, 143. Whitmarsh, A.J. and R.J. Davis 1998. Trends Biochem. Sci. 23, 481. Burack, W.R. and T.W. Sturgill 1997. Biochemistry 36, 5929. Mansour, S.J. 1996. Biochemistry 35, 15529.

Need additional information about this product? Email our Technical Service department at: technical@calbiochem.com

 Related information for this product is available:

EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Size
In Stock
Select Country Above
For Pricing Information
1 kitYN/A

Related Literature:

Biologics 32.2

Protein Kinase Assay and Detection Kits Brochure

Material Safety Data Sheets:
CBA030: PhosphoDetect™ MEK1 (pSer218/222) ELISA Kit - English
Bulgarian
Danish
Dutch
Finnish
French
German
Greek
Hungarian
Italian
Korean
Norwegian
Polish
Portuguese
Spanish
Swedish

Related Categories:
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Mitogen Activated Protein Kinase (MAPK)