| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse | | Sensitivity: ≤ 0.9 Units/ml | | Assay Range: 1.6-100 units/ml | | Assay Time: 3 h 20 min | | Sample Type: Cells | | Kit Contents: MEK1 (Ser218/222) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Detects and quantifies the level of MEK1 protein that is dually phosphorylated at Ser218 and Ser222. MEK1 is a member of the MEK family of dual specificity protein kinases which phosphorylates threonine and tyrosine residues on MAP kinases ERK 1 and 2. Activation of MEK1 is critically involved in a wide range of cellular processes including cell proliferation, differentiation, and apoptosis. This kit is designed for use with human and mouse cells. | | Ref.: Colanzi, A., et al. 2003. J. Cell Biol. 161, 27. Slack-Davis, J.K., et al. 2003. Cell Biol. 162, 281. Suthiphongchai, T., et al. 2003. Oncol. Res. 13, 253. Alpin, A.E., et al. 2001. J. Cell Biol. 153, 273. Lee, S.E., et al. 2001. J. Biol. Chem. 276, 49343. Xu, B.E. 2001. J. Biol. Chem. 276, 26509. Kolch, W., et al. 2000. Biochem. J. 351, 289. Bondzi, C., et al. 2000. Oncogene 19, 5030. Whitmarsh, A.J. and R.J. Davis 1999. Science's stke www.stke.org/cgi/content/full/OC_sigtrans; 1999/1/pe1. Widman, C., et al. 1999. Physiol. Rev. 79, 143. Whitmarsh, A.J. and R.J. Davis 1998. Trends Biochem. Sci. 23, 481. Burack, W.R. and T.W. Sturgill 1997. Biochemistry 36, 5929. Mansour, S.J. 1996. Biochemistry 35, 15529. | |