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Kinase Kit Resource
  c-Met ELISA Kit
Cat. No. CBA031  
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Protein Tyrosine Kinase (PTK)

Format: 96-well plate
Form: 96 Tests
Species Reactivity: human, mouse, rat
Sensitivity: <4 ng/ml
Assay Range: 0.78-50 ng/ml
Assay Time: 4 h
Sample Type: Cells
Kit Contents: Coated 96-Well Plate, c-Met Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.
Comments: Detects and quantifies the levels of c-Met protein independent of its phosphorylation state. The ligand for c-Met, HGF/SF, is a member of the plasminogen-related growth factor family, which is synthesized as an inactive pro-form. HGF/SF stimulation of c-Met expressing cells enhances proliferation, migration, morphogenesis, and protease synthesis, characteristics that are associated with invasive cell phenotype. This kit is designed for use with human samples.
Ref.: Kang, J.Y, et al. 2003. Cancer Res. 63, 1101. Zhang, X., et al. 2003. Am. J. Renal Physiol. 284, F82. Maulik, G., et al. 2002. Cytokine Growth Factor Rev. 13, 41. Crostella, L., et al. 2001. Oncogene 20, 3735. Fan, S., et al. 2001. Mol. Cell. Biol. 21, 4968. Longati, P., et al. 2001. Curr. Drug Targets 2, 41. Matsumoto, K. and T. Nakamura 2001. Kidney Int. 59, 2023. Parr, C., et al. 2001. Biochem. Biophys. Res. Commun. 285, 1330. Follenzi, A., et al. 2000. Oncogene 19, 3041. Furge, K.A., et al. 2000. Oncogene 19, 5582. Wei, L., et al. 1995. J. Biol. Chem. 270, 8122. Guiton, M., et al. 1994 J. Biol. Chem. 269, 30370. Park, M., et al. 1987. Proc. Nat'l. Acad. Sci. 84, 6379.

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Cat. No.Disclaimers
CBA031
Canadian Export RegulationsDue to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.


Detection of c-Met by ELISA vs Immunoblotting. The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Met. The data presented in Figure 1 show that the sensitivity of the ELISA is ~2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-c-Met antibody and chemiluminescent detection.

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Biologics 32.1

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CBA031: c-Met ELISA Kit - English
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All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Protein Tyrosine Kinase (PTK)