Format: 96-well plate

Form: 96 Tests

Species Reactivity: human

Sensitivity: < 0.9 Unit/ml

Assay Range: 1.6-100 units/ml

Assay Time: 4 h

Sample Type: Cells

Kit Contents: STAT1 (Tyr701) Standard, Diluents, Detection Antibody, Secondary Antibody, Sample Buffer, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.

Comments: Detects and quantifies the level of the STAT1 phosphorylated at Tyr701. STAT1 is a member of STAT family of proteins that phosphorylates at Tyr701 and is essential for normal transcriptional activity of ISGF3 (interferon-stimulated gene factor 3) complex. It participates in the regulation of cytokine-signaling and cellular responses and plays an important role in growth and differentiation in a variety of cell types. This kit is designed for use with human cells and tissues.

Ref.: Boudny, V. and J. Kovarik 2002. Neoplasm 49, 349.0 Brivanlou, A.H. and J.E. Darnell 2002. Science 295, 813. Bromberg, J. 2002. J. Clin. Invest. 109, 1139. Levy, D.E. and J.E. Darnell 2002. Nat. Rev. Mol. Cell. Biol. 3, 651. Ghislain, J.J., et al. 2001. Interferon Cytokine Res. 21, 379. Shankaran, V. and R.D. Shreiber 2001. In Cytokine Reference. Volume 2: Receptors. J.J. Oppenheim, M. Feldmann, S.K. Durum, T. Hirano, J. Vilcek, and N.A. Nicola, editors. Academic Press, London, UK. pp.1819. Stephens, J.M., et al. 1998. J. Biol. Chem. 273, 31408.