| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: < 0.5 ng/ml | | Assay Range: 0.94-60 ng/ml | | Assay Time: 4 h | | Sample Type: Cells | | Positive Control: IR (b-subunit) | | Kit Contents: IR (b-subunit) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Detects and quantifies the levels of insulin receptor (IR) b-subunit independent of its phosphorylation status. The IR is a cell surface receptor that belongs to the superfamily of the growth factor receptor tyrosine kinases and regulates multiple signaling pathways. Although this kit is designed for use with human cell lines, it cross-reacts with mouse and rat cells. | | Ref.: Bevan, P. 2001. J. Cell. Sci. 114, 1429. Skorey, K.I., et al. 2001. Anal. Biochem. 291, 269. Ottensmeyer, F.P. et al. 2000. Biochemistry 39, 12103. Playford, M.P., et al. 2000. Proc. Nat'l. Acad. Sci. USA 97, 12103. Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931. Wei, L., et al. 1995. J. Biol. Chem. 270, 8122. Ebina, Y., et al. 1985. Cell 40, 747. Ullrich, A., et al. 1985. Nature 313, 756. | |