| Format: Flow cytometry | | Form: 50 Tests | | Detection Method: Fluorescence | | Assay Time: 4-5 h | | Sample Type: Cells | | Kit Contents: Positive Control Cells, Negative Control Cells, Wash Buffer, Reaction Buffer, TdT, Rinsing Buffer, FITC dUTP, PI/RNase Staining Buffer, and a user protocol. | | Comments: A two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry or laser scanning cytometry. This kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. | | Ref.: Lockshin, R.A., 2001. Nat. Rev. Mol. Cell Biol. 2, 545. Gorczyca, W., et al. 1996. Cell Proliferation 25, 539. Li, X., eta al. 1995. Cytometry 20, 172. Nagata, S., and Golstein, P., 1995. Science 267, 1449. Steller, H. 1995. Science 267, 1445. Thompson, C. B. 1995. Science 267, 1456. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Goldman, A.S., 1983. Proc. Soc. Exp. Biol. Med. 174, 239. |
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