a-Secretase Activity Kit
ADAM17 Activity Kit
TNF-a Converting Enzyme Activity Kit

Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorometric

Assay Range: 5-100 ng/ml as measured with purified recombinant human TACE

Sample Type: Cells

Positive Control: Human recombinant TACE

Kit Contents: Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol.

Comments: The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.

Ref.: Kirkegaard, T., et al. 2004. Clin. Exp. Immunol. 135, 146. Neumann, U., et al. 2004. Anal. Biochem. 328, 166. Skovronsky, D.M., et al. 2001. J. Neurobiol. 49, 40. Black, R.A., et al. 1997. Nature 385, 729. Moss, M.L., et al. 1997. Nature 385, 733.

Not available for sale in Germany.