Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorometric

Assay Time: 14-26 h

Sample Type: Cells

Kit Contents: 96-Well Laminin-Coated 96-Well Cell Invasion Chamber, Black 96-Well Plate, Additional Tray , Calcein-AM Solution, Cell Detachment Buffer, and a user protocol.

Comments: The InnoCyte™ Laminin-Based 96-well Cell Invasion Assay is designed to determine the invasive capacity of cells using laminin as a significant barrier to invasion or to screen potential anti-metastatic agents in vitro in a convenient, high-throughput, 96-well format. The 8 mm pore size is appropriate for all types of adherent cells such as epithelial, endothelial, or fibroblast cells. The assay is based on the Boyden-chamber principle. Each well of the 96-well cell culture insert contains a polycarbonate membrane with an 8 mm pore size that is coated with a laminin protein layer. The laminin layer forms an effective barrier that prevents non-invasive cells from passing through the 8 mm pores. Invasive cells that can degrade the laminin layer will migrate through the membrane and attach to the underside of the membrane. The invasive cells are dislodged from the underside of the cell culture insert and stained with a fluorescent dye in a single step. Fluorescence is determined using a fluorimeter with an excitation wavelength of ~485 nm and an emission wavelength of ~520 nm.

Ref.: Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551. Ekblom, P., et al. 2003. Matrix Biol. 22, 35. Hood, J.D. and Cheresh, D.A. 2002. Nat. Rev. Cancer 2, 91. Aumailley, M., et al. 1998. J. Anat. 193, 1. Aumailley, M., et al. 1996. J. Invest. Dermatol. 106, 209. Burgeson, et al. 1994, Matrix Biol. 14, 209. Aznavoorian, S., et al. 1993. Cancer 71, 1368. Stetler-Stevenson, W. G., et al. 1993. Annu. Rev. Cell Biol. 9, 541.