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Kit Resource
  Active Caspase-3 ELISA Kit
Cat. No. CBA045  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse
Sensitivity: 0.1 ng/ml
Assay Time: 3.5 h
Sample Type: Cells
Kit Contents: Active Caspase-3 96-Well Plate, Active Caspase-3 Conjugate Concentrate, Type 12 Conjugate Diluent, Active Caspase-3 Standard, Calibrator Diluent RD5-20 Concentrate (5X), Extraction Buffer Concentrate (5X), Biotin-ZVKD-FMK Inhibitor, Wash Buffer Concentrate, Color Reagent A, Color Reagent B, Stop Solution, Plate Covers, and a user protocol.
Comments: Caspases belong to a family of cysteine proteases that cleave substrates at the carboxyl terminus of asparate residues. Active caspase-3 is one of the main caspases responsible for producing the morphological cellular changes known as apoptosis. This ELISA assay kit measures active caspase-3 in cell extracts.
Ref.: Saunders, P.A., et al. 2000. Anal. Biochem. 284, 114. Bradford, M.M. 1976. Anal. Biochem. 72, 248.

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Related Literature:

Biologics 32.2

Tools and Tips for Analyzing Apoptosis

Related Categories:
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase