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Kit Resource

Oxidative Stress Kits
  COX-2 ELISA Kit
Cat. No. CBA053  
All Categories » Calbiochem » Kits and Tools » Oxidative Stress Research Tools and Assays

Cyclooxygenase-2 ELISA Kit

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human
Assay Range: 2.5-200 ng/ml
Sample Type: Cell lysate, tissue extract
Positive Control: U937 cells
Kit Contents: Anti-COX-2 Coated 96-Well Plate, COX-2 Standard, COX-2 Detector Antibody, HRP Conjugate, Assay Diluent, ELISA 20X Plate Wash Concentrate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol
Comments: This kit detects and quantifies human COX-2 in cell lysates and tissue extracts. Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins from arachidonic acid. COX-2 expression induced by various inflammatory and/or mitogenic stimuli such as cytokines, growth factors or tumor promoters.
Ref.: Blanke, S.R., et al. 2005. Semin. Oncol. 32, 69. Dannenberg, A.J., et al. 2005. J. Clin. Oncol. 23, 254. Sanborn, R. and Blake, C.D., 2005. Semin. Oncol. 32, 69. Kim, H.J., et al. 2003. Int. J. Oncol. 22, 99. Shigemasa, K., et al. 2003. Int. J. Oncol. 22, 99. El-Bayoumy, K., et al. 2002. Int. J. Oncol. 20, 557. Hwang, D., et al. 1998. J. Natl. Cancer Inst. 90, 455.

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Standard Curve. Example standard curve using the COX-2 Standard. A standard curve was generated according to the COX-2 Standard preparation and the Detailed Protocol above. The results represent a typical standard curve. Assay range: 2.5-200 ng/ml. Lower limit of detection: ~2 ng/ml.

Related Literature:

Biologics 32.3

Material Safety Data Sheets:
CBA053: COX-2 ELISA Kit - English
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