Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorometric

Assay Range: 63-1000 ng/ml as measured with purified human calpain 1.

Sample Type: Cell lysates, tissues extracts

Positive Control: Calpain 1

Kit Contents: 96-Well Plate, Calpain 1 Control, Substrate, Assay Buffer, Activation Buffer, Inhibitor, Reducing Agent, Plate Sealer and a user protocol.

Comments: A highly sensitive and specific assay for measuring Calpain 1 and 2 activity in biological samples like cell lysate or tissue extracts and for screening enzyme inhibitors. Calpains (calcium ion-dependent papain-like cysteine proteases) are widely distributed in all mammalian cells. They mediate calcium-induced activation of the Erk1/Erk2 MAPK pathway and are believed to participate in intracellular signal processing via limited proteolysis of their targets.

Ref.: Higuchi, M., et al. 2005. J. Biol. Chem. 280, 15229; Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775; Veeranna, et al. 2004. Am. J. Pathol. 165, 795; Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296; Wang, K.K., et al. 2000. Trends Neurosci. 23, 20; Black, R.A., et al. 1997. Nature 385, 729; Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1; Sorimachi, H., et al. 1997. Biochem. J. 328, 721.