Format: Flow cytometry or fluorescence microscopy

Form: 20 Tests

Detection Method: Fluorescence

Assay Time: 30 min

Sample Type: Intact cells

Kit Contents: Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.

Comments: A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.

Ref.: Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1. Frey, T. 1997. Cytometry 28, 253; Boersma, A.W.M., et al. 1996. Cytometry 24, 123. White, E. 1996. Genes and Devel. 10, 1. Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545. Koopman, G., et al. 1994. Blood 84, 1415. Wyllie, A.H.,1993. Br. J. Cancer 67, 205. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Fadok, V.A., et al. 1992. J. Immunology 148, 2207. Schmid, I., et al. 1992. Cytometry 13, 204. Kerr, J.F.R., et al. 1972. Cancer 26, 239.