| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: < 0.9 Units/ml | | Assay Range: 1.6-100 Units/ml | | Unit Definition: One unit is defined as the amount of FAK (pTyr397) autophosphorylated from 300 pg total FAK. | | Assay Time: 4 h | | Sample Type: Cell lysate | | Positive Control: 3T3L1 cells | | Kit Contents: FAK (pTyr397) Standard, Standard Diluent Buffer, FAK Antibody-Coated 96-Well Plate, Rabbit Anti-FAK(pTyr397) Detector Antibody, Goat Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | | Comments: Detects and quantifies the level of FAK (Focal Adhesion Kinase) phosphorylated at Tyr397. FAK is a non-receptor tyrosine kinase that has been implicated in several cell processes including the regulation of cell motility, cell morphology and cell adhesion. | | Ref.: Crowe, D.L., et al. 2004. BMC Cancer 4, 18. Xu, L.H., et al. 2004. J. Biol. Chem. 275, 30597. Yu, H.G., et al. 2004. Biochem. Pharmacol. 67, 135. Lee, J.W., et al. 2003. J. Orthop. Res. 21, 1071. Streblow, D.N., et al. 2003. J. Biol. Chem. 278, 50456. Jones, G., et al. 2001. Cancer Res. 61, 4978. Ruest, P.J., et al. 2000. Cell Growth Differ. 11, 41. Igishi, T., et al. 1999. J. Biol. Chem. 274, 30738. Owen, J.D., et al. 1999. Mol. Cell. Biol. 19, 4806. Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677. Cary, L.A., et al. 1996. J. Cell Sci. 109, 1787. | |