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VEGF Signaling Pathway
  FAK ELISA Kit
Cat. No. CBA063  
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Protein Tyrosine Kinase (PTK)

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse, rat
Sensitivity: < 1.6 ng/ml
Assay Range: 1.6-100 ng/ml
Assay Time: 4 h
Kit Contents: FAK Standard, Standard Diluent Buffer, FAK Antibody-Coated 96-Well Plate, Rabbit Anti-FAK Detector Antibody, Goat Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.
Comments: Detects and quantifies the level of FAK (Focal Adhesion Kinase) protein independent of its phosphorylation state in human, mouse and rat cells. FAK is a non-receptor tyrosine kinase that has been implicated in several cell processes including the regulation of cell motility, cell morphology and cell adhesion.
Ref.: Crowe, D.L., et al. 2004. BMC Cancer 4, 18. Xu, L.H., et al. 2004. J. Biol. Chem. 275, 30597. Yu, H.G., et al. 2004. Biochem. Pharmacol. 67, 135. Lee, J.W., et al. 2003. J. Orthop. Res. 21, 1071. Streblow, D.N., et al. 2003. J. Biol. Chem. 278, 50456. Jones, G., et al. 2001. Cancer Res. 61, 4978. Ruest, P.J., et al. 2000. Cell Growth Differ. 11, 41. Igishi, T., et al. 1999. J. Biol. Chem. 274, 30738. Owen, J.D., et al. 1999. Mol. Cell. Biol. 19, 4806. Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677. Cary, L.A., et al. 1996. J. Cell Sci. 109, 1787.

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1 kit*$628.00*

* Additional Product Information
Cat. No.Disclaimers
CBA063-1KIT
Stock StatusPlease contact our Customer Service Department for delivery time.
CBA063
Canadian Export RegulationsDue to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.


Detection of FAK Using the FAK ELISA Kit. 3T3L1 cells were left untreated or treated with 1 mM sodium orthovandate for 5h. Cell lysates were analyzed using the FAK ELISA Kit. Phosphorylated and unphosphorylated FAK were detected equally.


Parallelism. 3T3L1 cell lysate was serially diluted in Standard Diluent Buffer and assayed for FAK using the Detailed Protocol. The absorbance of each dilution was plotted against the FAK standard curve. Parallelism demonstrated by the figure above indicated that the standard accurately reflects FAK content in samples.

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Biologics 32.3

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CBA063: FAK ELISA Kit - English
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All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Protein Tyrosine Kinase (PTK)