| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: <200 pg/ml | | Assay Range: 0.313-20 ng/ml | | Assay Time: 4 h | | Kit Contents: PRAS40 Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | | Comments: Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) independent of its phosphorylation state. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities. | | Ref.: Saito, A., et al. 2004. J. Neurosci. 24, 1584. Kovacina, K.S., et al. 2003. J. Biol. Chem. 278, 10189. Noshita, N., et al. 2003. Stroke 34, 1513. Cantley, L.C. 2002. Science 296, 1655. Lizcano, J.M. and Alessi, D.R. 2002. Curr. Biol. 12, R236. Macias, M.J., et al. 2002. FEBS Lett. 513, 30. Tzivion, G. and Avruch, J. 2002. J. Biol. Chem. 277, 3061. Cho, H., et al. 2001. J. Biol. Chem. 276, 38349. Noshita, N., et al. 2001. J. Cereb. Blood Flow Metabol. 21, 1442. Eves, E.M., et al. 1998. Mol. Cell Biol. 18, 2143. Fujio, Y., et al. 1999. Mol. Cell Biol. 19, 5073. Thorson, J.A., et al. 1998. Mol. Cell Biol. 18, 5229. | |