Return to our Home Page


Tech Resources
User Protocol
Certificate of Analysis
Store
2 to 8°C2 to 8°C
Ship
Shipped with Blue Ice
<br>
Shipped with blue ice (water ice)!Blue ice
Multiple Toxicity Values, refer to MSDSMultiple Toxicity Values, refer to MSDS
Note: Store and Ship conditions may differ.
See Key

Kit Resource
  PRAS40 ELISA Kit
Cat. No. CBA066  
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Akt (Protein Kinase B)

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse, rat
Sensitivity: <200 pg/ml
Assay Range: 0.313-20 ng/ml
Assay Time: 4 h
Kit Contents: PRAS40 Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol.
Comments: Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) independent of its phosphorylation state. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities.
Ref.: Saito, A., et al. 2004. J. Neurosci. 24, 1584. Kovacina, K.S., et al. 2003. J. Biol. Chem. 278, 10189. Noshita, N., et al. 2003. Stroke 34, 1513. Cantley, L.C. 2002. Science 296, 1655. Lizcano, J.M. and Alessi, D.R. 2002. Curr. Biol. 12, R236. Macias, M.J., et al. 2002. FEBS Lett. 513, 30. Tzivion, G. and Avruch, J. 2002. J. Biol. Chem. 277, 3061. Cho, H., et al. 2001. J. Biol. Chem. 276, 38349. Noshita, N., et al. 2001. J. Cereb. Blood Flow Metabol. 21, 1442. Eves, E.M., et al. 1998. Mol. Cell Biol. 18, 2143. Fujio, Y., et al. 1999. Mol. Cell Biol. 19, 5073. Thorson, J.A., et al. 1998. Mol. Cell Biol. 18, 5229.

Need additional information about this product? Email our Technical Service department at: technical@calbiochem.com

 Related information for this product is available:

EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Size
In Stock
Select Country Above
For Pricing Information
1 kitYN/A*

* Additional Product Information
Cat. No.Disclaimers
CBA066
Canadian Export RegulationsDue to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.


Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of PRAS40. The data show that the sensitivity of the ELISA is approximately 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-PRAS40 antibody and chemiluminescent detection.

Related Literature:

Biologics 32.4

Protein Kinase Assay and Detection Kits Brochure

Related Categories:
All Categories » Calbiochem » Kits and Tools » Protein Kinase Assays » Akt (Protein Kinase B)