Calbiochem: CBA067: PhosphoDetect™ PRAS40 (pThr246) ELISA Kit

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PhosphoDetect� Phospho-specific Antibodies

��	PhosphoDetect™ PRAS40 (pThr²⁴⁶) ELISA Kit

Cat. No. CBA067��

All Categories � Calbiochem � Kits and Tools � Protein Kinase Assays � Akt (Protein Kinase B)

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse, rat
Sensitivity: < 0.9 units/ml
Assay Range: 1.6-100 Units/ml
Assay Time: 4 h
Sample Type: Cell lysate, tissue extract
Positive Control: 3T3L1 cells
Kit Contents: PRAS40 (pThr²⁴⁶) Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 (pThr²⁴⁶) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.
Comments: Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) protein that is phosphorylated at Thr²⁴⁶ in mouse, human, and rat samples. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities.
Ref.: Saito, A., et al. 2004. J. Neurosci. 24, 1584. Kovacina, K.S., et al. 2003. J. Biol. Chem. 278, 10189. Noshita, N., et al. 2003. Stroke 34, 1513. Cantley, L.C. 2002. Science 296, 1655. Lizcano, J.M. and Alessi, D.R. 2002. Curr. Biol. 12, R236. Macias, M.J., et al. 2002. FEBS Lett. 513, 30. Tzivion, G. and Avruch, J. 2002. J. Biol. Chem. 277, 3061. Cho, H., et al. 2001. J. Biol. Chem. 276, 38349. Noshita, N., et al. 2001. J. Cereb. Blood Flow Metabol. 21, 1442. Fujio, Y., et al. 1999. Mol. Cell Biol. 19, 5073. Eves, E.M., et al. 1998. Mol. Cell Biol. 18, 2143. Thorson, J.A., et al. 1998. Mol. Cell Biol. 18, 5229.

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CBA067-1KIT	Stock Status	Please contact our Customer Service Department for delivery time.



CBA067	Canadian Export Regulations	Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of PRAS40 (pThr²⁴⁶). The data presented in Figure 1 show that the sensitivity of the ELISA is ~2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using rabbit anti-PRAS40 (pThr²⁴⁶) and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent substrate and autoradiography detection.

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All Categories � Calbiochem � Kits and Tools � Protein Kinase Assays � Akt (Protein Kinase B)

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