| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Sensitivity: < 0.26 ng/ml | | Assay Range: 0.39-25 ng/ml | | Assay Time: 4 h | | Kit Contents: GSK-3b Standard, Standard Diluent Buffer, GSK-3b Antibody-Coated 96-Well Plate, Rabbit Anti-GSK-3b Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | | Comments: Detects and quantifies the level of GSK-3b (Glycogen synthase kinase-3 beta) protein independent of its phosphorylation state in human, mouse and rat cells. GSK-3b is a non-receptor serine/threonine kinase that is a key modulator of cell fate, neuronal plasticity, and tumorigenesis. It also plays an important role in diabetes, stroke, and Alzheimer's disease via regulation of microtubule stability and phosphorylation of the microtubule associating protein, tau. | | Ref.: Bhatt, R.V., et al. 2004. J. Neurochem. 89, 1313. Kirshenboim, N., et al. 2004. J. Mol. Neurosci. 24, 237. Linseman, D.A., et al. 2004. J. Neurosci. 24, 9993. Cho, J.H. and Johnson, G.V. 2003. J. Biol. Chem. 278, 187. Doble, B.W. and Woodgett, J. R. 2003. J. Cell Sci. 116, 1175. Zhang, F., et al. 2003. J. Biol. Chem. 278 33067. Martinez, A., et al. 2002. J. Med. Chem. 45, 1292. Ali, A., et al. 2001. Chem. Rev. 101, 2527. Godemann, R., et al. 1999. FEBS Lett. 454, 157. |
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