Calbiochem: CBA069: PhosphoDetect™ GSK-3b (pSer9) ELISA Kit

Tech Resources
	User Protocol
	MSDS - English
	Other Languages
	Certificate of Analysis

Store
	2 to 8�C
Ship
	Blue ice
	Multiple Toxicity Values, refer to MSDS
Note: Store and Ship conditions may differ.
See Key

PhosphoDetect� Phospho-specific Antibodies

��	PhosphoDetect™ GSK-3b (pSer⁹) ELISA Kit

Cat. No. CBA069��

All Categories � Calbiochem � Kits and Tools � Protein Kinase Assays � Other

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human, mouse, rat
Sensitivity: < 0.4 U/ml
Assay Range: 1.6-100 U/ml
Unit Definition: One unit (U) is defined as the amount of GSK-3b (pSer⁹) phosphorylated in 300 pg total GSK-3b.
Assay Time: 4 h
Kit Contents: GSK-3b (pSer⁹) Standard, Standard Diluent Buffer, GSK-3b Antibody-Coated 96-Well Plate, Rabbit Anti-GSK-3b (pSer⁹) Detector Antibody, Goat Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol.
Comments: Detects and quantifies the level of GSK-3b (glycogen synthase kinase-3beta) phosphorylated at Ser⁹ in human, mouse, and rat cells. GSK-3b is a non-receptor serine/threonine kinase that is a key modulator of cell fate, neuronal plasticity, and tumorigenesis. It also plays an important role in diabetes, stroke, and Alzheimer's disease via regulation of microtubule stability and phosphorylation of the microtubule associating protein, tau.
Ref.: Bhatt, R.V., et al. 2004. J. Neurochem. 89, 1313. Kirshenboim, N., et al. 2004. J. Mol. Neurosci. 24, 237. Linseman, D.A., et al. 2004. J. Neurosci. 24, 9993. Cho, J.-H., et al. 2003. J. Biol. Chem. 278, 187. Doble, B.W. and Woodgett, J. R. 2003. J. Cell Sci. 116, 1175. Zhang, F., et al. 2003. J. Biol. Chem. 278, 33067. Martinez, A., et al. 2002. J. Med. Chem. 45, 1292. Ali, A., et al. 2001. Chem. Rev. 101, 2527. Godemann, R., et al. 1999. FEBS Lett. 454, 157.

Need additional information about this product? Email our Technical Service department at: technical@calbiochem.com

� Related information for this product is available:
Additional information is available from the "Tech Resources"
box in the upper left panel of this page.

EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Sales Office Contact Details

Size
In Stock
Select Country Above
For Pricing Information

1 kit Y N/A*

* Additional Product Information


Cat. No.	Disclaimers


CBA069	Canadian Export Regulations	Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

Linerearity of Dilution. Jurkat cells were grown in tissue culture medium containing 10% fetal calf serum were treated with 100 mM LiCl for 3 h and lysed with Cell Lysis Buffer. The lysate was diluted in Standard Diluent Buffer over the range of the assay and measured for GSK-3b (pSer⁹). Linear regression analysis of samples versus the expected concentration yielded a correlation coefficient of 0.99.

Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of GSK-3b (pSer⁹). The data show that the sensitivity of the ELISA is approximately 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed an anti-GSK-3b (pSer⁹) and chemiluminescent detection.

Material Safety Data Sheets:
CBA069: PhosphoDetect™ GSK-3b (pSer⁹) ELISA Kit - English
Bulgarian
Danish
Dutch
Finnish
French
German
Greek
Hungarian
Italian
Korean
Norwegian
Polish
Portuguese
Spanish
Swedish

Related Categories:
All Categories � Calbiochem � Kits and Tools � Protein Kinase Assays � Other

Change Country | Contact Us | Privacy Policy | Terms and Conditions

©2008 Calbiochem, Novabiochem, & Novagen

are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.