Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Species Reactivity: human, mouse

Specificity: Human and mouse CREB with slight cross-reactivity with CREM

Sensitivity: < 0.025 ng/ml

Assay Range: 0.156-10 ng/ml

Assay Time: 4 h

Sample Type: Cells

Positive Control: HeLa, Jurkat, HT1080, and NIH3T3 cells

Kit Contents: CREB Standard, Standard Diluent Buffer, CREB Antibody-Coated 96-Well Plate, Rabbit Anti-CREB (Total) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.

Comments: Detects and quantifies the level of CREB (cAMP-Response Element-Binding protein) independent of its phosphorylation state in human and mouse cells. CREB is a member of the leucine zipper family of DNA binding proteins. It induces the transcription of target genes in response to several stimuli such as peptide hormones and growth factors.

Ref.: Impey, S. and Goodman, R. H. 2001. Sci. STKE. 82, PE1. Seternes, O.M., et al. 1999 Mol. Endocrinol. 13, 1071. Haywitz, A.J., and Greenberg, M.E. 1999. Annu. Rev. Biochem. 68, 821. Xing, J., et al. 1998. Mol. Cell. Biol. 18, 1946. Maldonado, R., et al. 1996. Science 273, 611. Gonzales, G.A. and Montminy, M.R. 1989. Cell 59, 675.

R: 25-36/37/38; S: 26-36