 |
 | 2 to 8°C |  | Blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| | PhosphoDetect™ CREB (pSer133) ELISA Kit |
|  | Cat. No. CBA072 |
|
| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse | | Sensitivity: < 0.9 Units/ml | | Assay Range: 1.6-100 Units/ml | | Assay Time: 4 h | | Kit Contents: CREB (pSer133) Standard, Standard Diluent Buffer, CREB Antibody-Coated 96-Well Plate, Rabbit Anti-CREB (pSer133) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | | Comments: Detects and quantifies the level of CREB (cAMP-Response Element-Binding protein) phosphorylated at Ser133 in human and mouse cells. CREB is a member of the leucine zipper family of DNA binding proteins. It induces transcription of target genes in response to several stimuli such as peptide hormones and growth factors. | | Ref.: Impey, S. and Goodman, R. H. 2001. Sci. STKE. 82, PE1. Haywitz, A.J., and Greenberg, M.E. 1999. Annu. Rev. Biochem. 68, 821. Seternes, O.M., et al. 1999 Mol. Endocrinol. 13, 1071. Xing, J., et al. 1998. Mol. Cell. Biol. 18, 1946. Maldonado, R., et al. 1996. Science 273, 611. Gonzales, G.A. and Montminy, M.R. 1989. Cell 59, 675. |
|
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
* Additional Product Information
Assay Specificity. HeLa cells were treated with 200 µM Forskolin for 20 min at 37°C. Untreated HeLa cells were used as control. All lysates were prepared and analyzed using the CREB (pSer133) ELISA Kit and CREB ELISA Kit. The total CREB remained comparable while the level of phosphorylated CREB increased after Forskolin treatment.
CREB (pSer133). Natural CREB (pSer133) from Forskolin-treated HeLa cells was serially diluted in Standard Diluent Buffer. The absorbance of each dilution was plotted against the CREB (pSer133) standard curve. Parallelism was demonstrated by the figure above and indicated that the standard accurately reflects CREB (pSer133) content in samples.
Dilution of CREB (pSer133) Standard.
Guidelines for diluting Anti-Rabbit IgG horseradish Peroxidase (HRP).
Peptide Blocking. The specificity of this assay for phosphorylated CREB (pSer133) was confirmed by peptide competition. The data show that the phosphopeptide containing the phosphorylated Ser133 blocks the ELISA signal. Non-phosphorylated peptide does not block the signal.
Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of CREB (pSer133). The data show that the sensitivity of the ELISA is approximately 4x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using rabbit anti- CREB (pSer133) and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent detection.
|
| | |
|
|
