Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Species Reactivity: human

Sensitivity: <0.25 Units/ml

Assay Range: 1.56-100 Units/ml

Unit Definition: One unit is defined as the amount of c-Met (pTyr1230/1234/1235) derived from 21,000 GTL 16 cells treated with 1 mM sodium orthovanadate for 16 h.

Assay Time: 4 h

Sample Type: Cells

Positive Control: A549 cells

Kit Contents: c-Met (pTyr1230/1234/1235) Standard, Standard Diluent Buffer, c-Met Antibody-Coated 96-Well Plate, Rabbit Anti-c-Met (pTyr1230/1234/1235) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol.

Comments: Detects and quantifies the level of c-Met phosphorylated at Tyr1230/1234/1235. c-Met is a proto-oncogene and a cell-surface receptor for hepatocyte growth factor, which plays an important role in signal transduction process. It is overexpressed in many cancers and often amplified between tumorigenesis and metastasis, so it could be a useful prognostic marker.

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