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 | 2 to 8°C |  | Blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. CBA074 |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, not mouse, not rat | | Sensitivity: < 15 pg/ml | | Assay Range: 19.5 - 1250 pg/ml | | Assay Time: 4 h | | Sample Type: Cell lysate | | Kit Contents: c-Kit Standard, Standard Diluent Buffer, c-Kit Antibody-Coated 96-Well Plate, Rabbit Anti-c-Kit Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | | Comments: Detects and quantifies the level of c-Kit protein independent of its phosphorylation state in human cells. c-Kit is a member of the receptor tyrosine kinase subfamily that includes PDGF, CSF-1, and FLT-3/flk-2 receptors. It plays a critical role in controlling a variety of cellular functions in hematopoietic stem cells, mast cells, melanocytes, and germ cells. | | Ref.: Fiedler, W., et al. 2003. Blood 102, 2763; Mol, C.D., et al. 2003. J. Biol. Chem. 278, 31461; Pardanani, A., et al. 2003. Lancet. 362, 535; Liang, X., et al. 2002. J. Biol. Chem. 277, 13732; Demetri, G.D. 2001. Semin. Oncol. 28, 19; Taylor, M.L., and Metcalfe, D.D. 2000. Hematol. Oncol. Clin. North Am. 14, 517; Lam, P.Y., et al. 1999. Biochem. J. 338, 131; Krystal, G.W., et al. 1997. Cancer Res. 57, 2203; Blume-Jensen, P., et al. 1995. J. Biol. Chem. 270, 14192. |
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* Additional Product Information
Effect of SFC treament on c-Kit (pTyr823) expression in Mo7e cells. Mo7e cells were treated with Stem Cell Factor (SCF) at varying concentrations (1 to 100 ng/ml) for 10 min, lysed, and quantitated in parallel for total c-Kit and c-Kit (pTyr823) expression. The amount of total c-Kit remains relatively constant, while the level of phosphorylation at Tyr823 increases with the dose of SCF. The results correlate very well with immunoblot of the same samples (inset).
Sensitivity. The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Kit. The data show that the sensitivity of the ELISA is approximately 4X greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-c-Kit antibody and chemiluminescent detection.
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