Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorescence

Assay Range: 50-1000 ng/ml

Assay Time: 3.5 h

Sample Type: Cell lysates, tissue extracts, biological fluids

Positive Control: Recombinant rat IDE

Kit Contents: Control Rat Recombinant IDE, Anti-Insulysin/IDE Coated 96-Well Plate, Substrate, Assay Buffer, 10X Sample Buffer, Plate Sealer, and a user protocol.

Comments: A specific and sensitive assay for measuring active human, mouse, or rat Insulysin (IDE) in cell lysates, tissue extracts, and biological fluids. IDE is a major enzyme responsible for degrading insulin hence critical for glucose, lipid, and protein metabolism, as well as for cell growth and differentiation. It also plays an important role in both diabetes and Alzheimer's disease.

Ref.: Li, Q., et al. 2006. Cell 127, 305. Shen, Y., et al. 2006. Nature 443, 870. Song, E.S. and Hersh, L.B., 2005. J. Mol. Neurosci. 25, 201. Farris, W., et al. 2003. PNAS USA 100, 4162. Morelli, L., et al. 2003. J. Biol. Chem. 278, 23221. Song, E.S., et al. 2001. J. Biol. Chem. 276, 1152.