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 | Multiple Storage Requirements |  | Blue ice |  | Irritant | | Note: Store and Ship conditions may differ. | | See Key |
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| | InnoCyte™ 96-well Cell Adhesion Array |
|  | Cat. No. CBA083 |
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| Format: 96-well plate | | Form: 96 Tests (14 samples x 6 ECM proteins and 12 negative controls) | | Detection Method: Fluorometric | | Kit Contents: ECM Protein-Coated 96-Well Plate Strips, Calcein-AM Solution, D-PBS, and a user protocol. | | Comments: A cell adhesion array intended for the determination of the relative attachment of adherent cell lines to 6 different ECM proteins-laminin, fibronectin, vitronectin, and collagen type I, type III, and type IV. | | Ref.: Stupack, D.G. 2005. Cell Death and Differentiation 12, 1021. Ekblom, P., et al. 2003.Matrix Biology 22, 35. Li, S., et al. 2002. J. Cell Biol. 157, 1279. Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588. Aumailley, M., et al. 1998. J. Anat. 193, 1. Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104. Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460. Frisch, S.M. 1994. J.Cell Biol. 124, 701. Meredith J.E. 1993. Mol. Biol. Cell 4, 953. | | R: 36/38; S: 26 |
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* Additional Product Information
ECM Proteins and the Corresponding Integrin Receptors.
ECM Proteins and the Corresponding Integrin Receptors.
Inhibition of HT-1080 Cell Attachment to Laminin I. Approximately 10,000 cells were added to wells coated with laminin I in the presence or absence of inhibitory antibody, anti-integrin b1 (Cat. No. CP26). The cells were incubated and labeled and fluorescence was measured as outlined in Figure 1.
Relative Attachment to Collagens. Approximately 30,000 cells were added to wells containing collagen I, III, or IV, and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO2. Fluorescence was measured as outlined in the Detailed Protocol.
Relative Cell Attachment of Human Cell Lines. Approximately 30,000 cells were added to wells containing fibronectin, laminin I, collagen type IV, vitronectin, basement membrane complex, and BSA and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO2. Fluorescence was measured as outlined in the Detailed Protocol.
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