Host: Mouse

Isotype: IgG2a

Immunogen: a synthetic peptide corresponding to amino acids 1-10 of the a-smooth muscle isoform of actin acetylated at the N-terminus and conjugated to KLH at the C-terminus

Form: Liquid

Formulation: In 10 mM PBS, 0.02% BSA, pH 7.4.

Preservative: ≤0.1% sodium azide

Positive Control: Vascular smooth muscle cells or blood vessels

Negative Control: Fibroblasts, striated muscle, or myocardium

Comments: Recognizes the ~42 kDa a-actin protein found in smooth muscle cells and pericytes in blood vessel walls, gut wall and myometrium.

Ref.: Ueyama, H., et al. 1990. Jap. J. Hum. Genet. 35, 145. Bergsma, D.J., et al. 1985. Mol. Cell. Biol. 5, 1151. Gunning, P., et al. 1984. PNAS 81, 1813. Lin, J.J.-C., et al. 1984. Monoclonal antibodies to cytoskeletal proteins. In: Monoclonal antibodies and functional cell lines. (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp. Vandekerckhove J., et al. 1984. J. Mol. Biol. 179, 391. Vandekerckhove J., et al. 1981. FEBS Lett. 113, 595. Lin, J.J.-C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46 769. Weber, K. 1978. J. Mol. Biol. 126, 783. Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York, 1976.