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| | PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) |
|  | Cat. No. DR1002 |
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Anti-Ataxia Telangiectasia, Phospho-Specific (Ser1981) | Host: Mouse | | Isotype: IgG1 | | Immunogen: a synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser1981 phosphorylation site of human ATM | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa cells treated with camptothecin (Cat. No. 208925) | | Comments: Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992. | | Ref.: Horejsi, Z., et al. 2004. Oncogene 23, 3122. Abraham, R.T. 2003. Genes Dev. 15, 2177. Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499. Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155. | | Sold under license of U.S. Patent 7,108,992. |
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| 10H11.E12 | human, mouse | IB, IC
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Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.
Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
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