 |
 | -20°C |  | Blue ice | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| | Anti-p53 Binding Protein 1 Mouse mAb (BP13) |
|  | Cat. No. DR1003 |
|
Anti-53BP1 | Host: Mouse | | Isotype: IgG1 | | Immunogen: a recombinant protein consisting of human p53BP1 fused to GST | | Form: Liquid | | Formulation: Undiluted ascites. | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa cell nuclear extract (Cat. No. WB64) or HEK293 whole cell lysate | | Comments: Recognizes the ~230 kDa 53BP1 protein in HeLa nuclear extract (Cat. No. WB64) and HEK293 cells. | | Ref.: Rappold, I., et al. 2001. J. Cell Biol. 153, 613. Ward, I.M., et al. 2001. J. Biol. Chem. 276, 47759. |
|
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. GBP list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| BP13 | human
not mouse | IB, IC, IP
See Key |
Detection of human 53BP1 by immunoblotting. Sample: Whole cell lysate (25 µg) from HEK293 cells. Primary antibody: Anti-p53 Binding Protein 1 Mouse mAb (BP13) (Cat. No. DR1003) (1:1000). Detection: chemiluminescence.
Detection of human 53BP1 by immunofluorescence. Samples: Untreated HeLa cells (left panel) or camptothecin-treated (12 ng/ml, 3 hr) HeLa cells, fixed with 1% paraformaldehyde and permeabilized with 0.2% Triton®-X 100 detergent (right panel). Primary antibody: Anti-p53 Binding Protein 1 Mouse mAb (BP13) (Cat. No. DR1003) (1:300). Detection: fluorescence with counterstain (blue).
|
|
|
