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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. DR1016 |
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Anti-Histone H2AX | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids at the C-terminus of human H2AX | | Form: Liquid | | Formulation: In Tris/citrate-phosphate buffer, pH 7-8. | | Preservative: ≤0.1% sodium azide | | Positive Control: Nuclear extracts from human 293T or G-361 cells | | Comments: Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form in 293T and A431 cell nuclear extracts. | | Ref.: Stiff. T., et al. 2004. Cancer Res. 64, 2390. Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273. Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579. Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858. |
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Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
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