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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| | PhosphoDetect™ Anti-H2AX (pSer139) Rabbit pAb |
|  | Cat. No. DR1017 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser139 phosphorylation site of human H2AX | | Form: Liquid | | Formulation: In Tris/citrate-phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: HEK293 cell nuclear extract | | Comments: Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form phosphorylated at Ser139. | | Ref.: Stiff. T., et al. 2004. Cancer Res. 64, 2390. Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273. Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579. Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858. |
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Detection of human and mouse g-H2AX, phosphorylated at Ser139 by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, g-H2AX embryonic fibroblasts (+/+), and mouse g-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-g-H2AX (pSer139) Rabbit pAb (1:10,000). Detection: chemiluminescence.
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