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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. DR1022 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids near the N-terminus of human Pso4 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: Jurkat cells | | Comments: Recognizes the ~55 kDa Pso4 protein. May immunoprecipitate an additional protein of ~110 kDa. | | Ref.: Mahajan, K.N., and Mitchell, B.S. 2003. Proc. Natl. Acad. Sci. USA 100, 10746. Grey, M., et al. 1996. Nucleic Acids Res. 24, 4009. |
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Left Panel: Detection of human Pso4 by immunoblotting. Sample: Whole cell lysate (40 µg) from Jurkat cells. Primary antibody: Anti-Pso4 Rabbit pAb (Cat. No. DR1022) (1:1000). Detection: chemiluminescence. Right Panel: Detection of human Pso4 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells. Antibody for IP: Anti-Pso4 Rabbit pAb (Cat. No. DR1022) (1:100). Immunoblotting conditions: same as above.
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