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| | PhosphoDetect™ Anti-p53 (pSer20) Rabbit pAb |
|  | Cat. No. DR1023 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser20 phosphorylation site of human p53 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Lysates from COS cells treated with UV | | Comments: Recognizes the ~53 kDa p53 protein phosphorylated at Ser20. Does not recognize unphosphorylated p53. | | Ref.: Xie, S., et al. 2001. J. Biol. Chem. 276, 36194. Chehab, N.H., et al. 2000. Genes Dev. 14, 278. Chehab, N.H., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 13777. |
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Detection of monkey p53, phosphorylated at Ser20, by immunoblotting: Samples: Whole cell lysate from COS cells treated with UV or methyl methanesulfonate (MMS) for the indicated times. Primary antibodies: PhosphoDetect™ Anti-p53 (pSer20) Rabbit pAb (Cat. No. DR1023) (1:1000) (upper panel) and Anti-p53, total (lower panel).
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