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| | PhosphoDetect™ Anti-p53 (pSer46) Rabbit pAb |
|  | Cat. No. DR1024 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser46 phosphorylation site of human p53 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Etoposide-treated MCF7 cells | | Comments: Recognizes the ~53 kDa p53 kDa protein phosphorylated at Ser46 in etoposide-treated MCF-7 cells. Does not recognizes unphosphorylated p53. | | Ref.: Wang, C., and Chen, J. 2003. J. Biol. Chem. 278, 2066. D'Orazi, G., et al. 2002. Nat. Cell Biol. 4, 11. Hoffman, T.G., et al. 2002. Nat. Cell Biol. 4, 1. Bulavin, D.V., et al. 1999. EMBO J. 18, 6845. |
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Detection of human p53, phosphorylated on Ser46, by immunoblotting. Sample: Whole cell lysate from MCF-7 cells treated with eptoposide (Cat. No. 341205). Primary antibody: Anti-p53, Phospho-Specific (Ser46) (Cat. No. DR1024) (1:1000). Detection: chemiluminescence.
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